Implementation of Quality by Design for the Development and Validation of Pioglitazone Hydrochloride by RP-UPLC with Application to Formulated Forms

Figure 4

Chromatograms obtained for method development using Acquity BEH C18, (100 × 2.1) mm, 1.7 μm column.

(a) ACN : Buffer (pH 3.2) (20 : 80% v/v)

(b) ACN : Water (20 : 80% v/v)

(c) Methanol : Water (20 : 80% v/v)

(d) Methanol : Buffer (pH3.2) (20 : 80% v/v)