Implementation of Quality by Design for the Development and Validation of Pioglitazone Hydrochloride by RP-UPLC with Application to Formulated Forms

Table 1

Observation and remarks of method development for Acquity BEH C8, (100 × 2.1) mm, 1.7 μm Column.

S. No.	Trails taken	Observations	Remarks
1	ACN : buffer (pH 3.2) (20 : 80% v/v)	Asymmetrical peak with fronting and tailing	Not Satisfactory
2	ACN : water (20 : 80% v/v)	Asymmetrical peak with tailing	Not Satisfactory
3	Methanol : water (20 : 80% v/v)	Split peak	Not Satisfactory
4	Methanol : buffer (pH 3.2) (20 : 80% v/v)	Asymmetrical peak	Not Satisfactory