Implementation of Quality by Design for the Development and Validation of Pioglitazone Hydrochloride by RP-UPLC with Application to Formulated Forms

Table 3

Observation and remarks of method development for different pH conditions with Acquity BEH C18, (100 × 2.1) mm, 1.7 μm column.

S. No.	Trails taken	Observations	Remarks	Theoretical plates	Tailing; factor
1	ACN : buffer (pH 3.2) (20 : 80% v/v)	Peaks found symmetrical	Satisfactory	3330	1.02
2	ACN : buffer (pH 4) (20 : 80% v/v)	Peak eluted early with less theoretical plates	Not satisfactory	1291	1.03
3	ACN : buffer (pH 5) (20 : 80% v/v)	Completely split peak	Not satisfactory	750	2.12
4	ACN : buffer (pH 6) (20 : 80% v/v)	Completely split peak	Not satisfactory	800	3.89