Table of Contents
ISRN Analytical Chemistry
Volume 2012, Article ID 609706, 5 pages
http://dx.doi.org/10.5402/2012/609706
Research Article

Validated HPTLC Method for Simultaneous Estimation of Atenolol and Aspirin in Bulk Drug and Formulation

Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth University, Maharashtra, Pune 411038, India

Received 10 October 2011; Accepted 20 November 2011

Academic Editors: W. Misiuk and K. Ohyama

Copyright © 2012 Vidhya K. Bhusari and Sunil R. Dhaneshwar. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This paper describes a new, simple, precise, and accurate HPTLC method for simultaneous estimation of Atenolol and Aspirin as the bulk drug and in tablet dosage forms. Chromatographic separation of the drugs was performed on aluminum plates precoated with silica gel 60 F254 as the stationary phase and the solvent system consisted of n-butanol : water : acetic acid (8 : 2 : 0.2 v/v/v). Densitometric evaluation of the separated zones was performed at 235 nm. The two drugs were satisfactorily resolved with R F values 0 . 2 3 ± 0 . 0 2 and 0 . 7 2 ± 0 . 0 2 for Atenolol and Aspirin, respectively. The accuracy and reliability of the method was assessed by evaluation of linearity (100–600 ng/spot for Atenolol and Aspirin), precision (intraday % RSD was 0.48–1.03 and interday % RSD was 0.68–1.14 for Atenolol, and intraday % RSD was 0.61–1.03 and interday % RSD was 0.69–1.04 for Aspirin), accuracy ( 1 0 0 . 1 7 ± 0 . 3 2 for Atenolol and 9 9 . 7 3 ± 0 . 5 1 for Aspirin), and specificity in accordance with ICH guidelines.