Table of Contents
ISRN Agronomy
Volume 2012 (2012), Article ID 617081, 8 pages
http://dx.doi.org/10.5402/2012/617081
Research Article

Effect of Stem Rust on the Quality of Selected Barley Genotypes

1Department of Crops Horticulture and Soils, Egerton University, P.O. Box 536, Egerton, Kenya
2Department of Biological Sciences, Egerton University, P.O. Box 536, Egerton, Kenya
3National Plant Breeding Centre, Kenya Agricultural Research Institute, P.O. Box Private Bag, Njoro, Kenya

Received 1 December 2011; Accepted 11 January 2012

Academic Editor: I. Vasilakoglou

Copyright © 2012 K. E. Mwando et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Stem rust, (Puccinia graminis f.sp. tritici) is a catastrophic disease of barley. Previously stem-rust-tolerant genes were incorporated in genotypes of wheat grown in Kenya, but in 1999, a new race Ug99 that is virulent to the genes was detected in Uganda. The new race spread rapidly and, in 2001, stem rust Ug99 isolates were detected in Kenya. Although barley is an important crop affected by stem rust, limited work has been done to it. In this study 20 barley cultivars from Kenya and from International Centre for Agriculture Research in Dry Area (ICARDA) were screened in greenhouse environment and in the field with isolate of Puccinia graminis f.sp. tritici. At seedling stage, the infection levels ranged from 0 to 2, except in ICARDA-09 and ICARDA-11 that showed infection types 3 and 3, 4, respectively. At adult plant stage, genotypes ICARDA-01, Nguzo, and Karne were moderately resistant while the rest were susceptible or moderately susceptible. In the field, the new line 1512-5 showed the highest severity of 93% in season 1, with Sabini having the highest severity of 30% in the second season. The highest reduction in percent germination (54.1% and 38.3%) was recorded in 1385-13 and ICARDA-10 in season 1 and 2, respectively. The highest loss (9.00%) in protein content was observed in Sabini in season 1. It is evident that stem rust Ug99 is a threat to beer industry and there is need for regular monitoring of the stem rust pathogen, in particular isolates in the variable Ug99 lineage, as well as continued resistance breeding. The study has demonstrated the pathogenicity of PgtUg99 to barley despite the fact that it poses a great threat to wheat production in the world.