Table of Contents
ISRN Dentistry
Volume 2012, Article ID 617245, 5 pages
Clinical Study

Lymphotoxin-Alpha Gene Polymorphism +252A/G (rs909253, A/G) Is Associated with Susceptibility to Chronic Periodontitis: A Pilot Study

1Division of Histology and Embryology, School of Biomedicine, Federal University of Piauí, 64049-550 Parnaíba, PI, Brazil
2Division of Histology and Embryology, Federal University of Campina Grande, 58109-990 Patos, PB, Brazil
3Department of Morphology, Division of Histology, School of Dentistry at Piracicaba, University of Campinas, 13083-970 Piracicaba, SP, Brazil
4Department of Morphology, Division of Histology, Institute São Leopoldo Mandic, 13045-610 Campinas, SP, Brazil
5Division of Histology and Embryology, School of Physiotherapy, Federal University of Piauí, 64049-550 Parnaíba, PI, Brazil
6Center for Oral and Systemic Diseases, Department of Periodontology, UNC School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7450, USA

Received 26 July 2012; Accepted 26 August 2012

Academic Editors: C. Lekic and A. Vissink

Copyright © 2012 Daniel Fernando Pereira Vasconcelos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. Periodontal disease leading to clinical findings such as increased periodontal probing depth involves a complex interaction between invading pathogenic microorganisms and the patient's immune system. Lymphotoxin alpha (LT-α) is a potent multifunctional immune modulator that contributes toward susceptibility to immune regulation disorders, including periodontal disease. Objective. In this study, we tested the hypothesis that chronic periodontitis (CP) is associated with polymorphisms of the LT-α gene. Materials and Methods. A total of 126 subjects, 44 healthy subjects, and 82 subjects with CP, were evaluated for periodontal disease by measuring clinical attachment loss and separation. Samples of epithelial cells were obtained for DNA analysis by scraping of the buccal mucosa. The LT-α gene was analyzed by polymerase chain reaction followed by endonuclease digestion with NcoI to analyze restriction fragment length polymorphisms. Results. The LT-α gene (+252A/G) polymorphism was associated with CP. LT-α allele frequencies were significantly different ( ) between patients with CP and healthy individuals, with an odds ratio of 2.67 for patients with CP with the G allele. Conclusions. These findings suggest the LT-α gene genotype is a risk indicator for susceptibility to chronic periodontal disease in the Brazilian population studied.