Use of Bacterial Artificial Chromosomes in Baculovirus Research and Recombinant Protein Expression: Current Trends and Future Perspectives
Figure 3
Iterative modification of AcMNPV to insert multiple single-locus expression cassettes. (a) Strategy used for repeated modification of the same bacmid to express multiple different recombinant proteins. ET recombination is targeted by viral flanking sequences (VF) to homologous sequences in the bacmid. Selection in E. coli is achieved using a bipartite marker consisting of a Zeocin resistance () and LacZ (Z), flanked by partially defective loxP sites. Following Cre-mediated recombination, this marker is removed, allowing a subsequent modification of the same DNA. (b) Loci within the AcMNPV successfully modified using the iterative modification strategy.
