Use of Bacterial Artificial Chromosomes in Baculovirus Research and Recombinant Protein Expression: Current Trends and Future Perspectives

Table 1

Summary of null mutants in AcMNPV generated in BACmid DNA by ET recombination.

Virus function	Gene deleted	References
DNA replication	helicase (p143), lef3, lef5, lef6, lef11, alk-exo, dna-pol	[70, 71, 94–100]
Transcription	ie-0 and ie-1	[101]
Cell entry	ac23,  gp64	[47, 102, 103]
Infectivity in insects	ac96, p74	[104, 105]
Nucleocapsid assembly	p6.9, ac53, vlf-1, 38K, ac101, ac142, ac144, ac109	[106–112]
Nucleocapsid release from the nucleus	exon0, ac66, vp80	[22, 85, 113–115]
Formation of enveloped virus	ac142, p48, ac76, odv-e18, odv-e56	[47, 116–118]
Interaction with host	egt, chiA, v-cath, IAP2	[47]
Unknown, essential genes	ac92, ac-fgf, ac11,  ac-pe	[47, 119]
Unknown, non-essential genes	me53, ac18,  ac-ctx	[47, 120, 121]