The Escherichia coli GcvB sRNA Uses Genetic Redundancy to Control cycA Expression
Figure 2
(a) WT terminator t1 and changes in t1 predicted to increase (↑) transcription termination. Primers used to construct the t1↑ allele delete the sequence distal to the t1↑ changes. (b) Northern analysis of GcvB. RNA was isolated from WT, ΔgcvB, or ΔgcvB transformed with a single-copy plasmid carrying the allele and probed with either a DIG-labeled GcvB or 5S rRNA-specific DNA probe. Numbers above each lane indicate levels of GcvB relative to WT.
