Development and Stability Studies of Novel Liposomal Vancomycin Formulations

Table 2

Stability of conventional and PEGylated vancomycin liposomes at the end of 1, 2 and 3 months.

Formulation	Test condition	Temp	% EE	Particle size (nm)	Physical appearance
Conventional liposomes	0 Mon		12.40	234±92	Milky suspension
	1 Mon	4°C	11.71	258±111	Milky suspension
		24°C	8.68	242±97	Milky suspension; slight coagulation
		37°C	4.28	605±800	Slightly dark color and thicker suspension, with growth of fungus on top, dispersed upon shaking.
	2 Mon	4°C	8.47	260±85	Milky suspension
		24°C	5.01	305±112	Slight dark color suspension with formation of big clumps of fungus, which are not dispersible upon shaking.
	3 Mon	4°C	8.23	250±97	Milky suspension
		24°C	3.67	356±43	Slight dark color suspension with formation of big clumps of fungus, which are not dispersible upon shaking
PEGylated liposomes	0 Mon		12.78	181±82	Milky suspension
	1 Mon	4°C	11.17	202±107	Milky suspension
		24°C	9.40	200±106	Milky suspension; slight coagulation
		37°C	6.90	208±109	Slightly dark color and thicker suspension, with growth of fungus on top, dispersed upon shaking.
	2 Mon	4°C	10.33	205±93	Milky suspension
		24°C	5.29	355±154	Slight dark color suspension with formation of big clumps of fungus, which are not dispersible upon shaking.
	3 Mon	4°C	9.89	210±88	Milky suspension
		24°C	4.98	290±120	Slight dark color suspension with formation of big clumps of fungus, which are not dispersible upon shaking