Research Article
Assessment of Pseudoaffinity Chromatography Using Textile Dyes for Isolation of Buffalo Pituitary Luteinizing Hormone
Table 1
Quantitative elution pattern of protein on different dye-agarose columns.
| S. no. | Name of the dye | Changing the composition of the buffers (%) | Changing the pH of the buffers (%) | Changing the ionic strength of the buffers (%) |
| (1) | Cibacron blue 3GA | 70.46 | 79.03 | 84.94 | (2) | Reactive blue | 53 | 61.13 | 80.52 | (3) | Reactive brown | 47.52 | 55 | 87.64 | (4) | Reactive yellow | 95 | 96.1 | 96.76 | (5) | Reactive red | ā | 39.76 | 49.82/92.7 (arginine) | (6) | Reactive green | 40.48 | 74 | 77.94 |
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