Upregulation of TLR1, TLR2, TLR4, and IRAK-2 Expression During ML-1 Cell Differentiation to Macrophages: Role in the Potentiation of Cellular Responses to LPS and LTA
Figure 2
Expression of TLR mRNA expression in ML-1 cells. Six million cells were incubated in the presence TPA of (5 nM) or vehicle (DMSO) for indicated times. Total RNAs were extracted from cell samples using Trizol reagents, purified with the Qiagen RNAeasy mini kit and analyzed by microarray analysis using human Genome U133 2.0 PlusArray Chip. Six replicates including three controls (DMSO-treated) and three TPA-treated ML-1 cells samples were examined. The levels of (a) TLR1, TLR2, TLR4 and TLR8 and (b) IRAK-2 mRNAs were determined by examination of the microarray analysis data.
