(a) A multiple amino acid alignment of 9 representative bacterial azurins and 3 azurin-like proteins (Laz) from neisserial species. Identical residues (ā), conservative amino acid substitutions (:), and semiconservative amino acid substitutions (.) are shown below the aligned sequences. Laz and azurins that were used included are the following: Laz from N. gonorrhoeae FA 1090 (YP_208090.1); H.8 outer-membrane lipoprotein from N. meningitidis WUE 2594 (CBY91147.1); azurin from N. lactamica ATCC 23970 (ZP_05986830.1); azurin from Bordetella pertussis Tohama I (NP_879636.1); azurin from Achromobacter xylosoxidans AXX-A (EGP47536.1); azurin from P. aeruginosa PAO1 (NP_253609.1); azurin from Pseudomonas syringae pv. glycinea str. race 4 (EGH06424.1); azurin from Achromobacter xylosoxidans (BAA33677.1); azurin from Burkholderia cepacia (AAP03090.1); azurin from Pseudomonas mendocina ymp (YP_001186158.1); azurin from Bordetella parapertussis 12822 (NP_885568.1) and azurin from Vibrio parahaemolyticus 10329 (EGF40167.1). The CLUSTAL X software [29] was used to generate this multiple sequence alignment. (b) A phylogenetic tree calculated from the alignment represented above in (a). Clustal X with neighbour-joining method [30] was used to construct the tree.
