Table of Contents
ISRN Spectroscopy
Volume 2012 (2012), Article ID 643979, 9 pages
http://dx.doi.org/10.5402/2012/643979
Research Article

Highly Sensitive High Performance Liquid Chromatography-Laser Induced Fluorescence for Proteomics Applications

1Centre for Atomic and Molecular Physics, Manipal University, Manipal 567104, India
2Department of Microbiology, KMC International Center, Manipal 567104, India
3Department of Oral Medicine and Radiology, Manipal College of Dental Sciences, Manipal 567104, India
4Udayagiri, Deonar, Sion, Mumbai 400 088, India

Received 9 May 2012; Accepted 27 June 2012

Academic Editors: A. A. Ensafi, A. Huczynski, and B. Liu

Copyright © 2012 Ajeetkumar Patil et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This paper describes the sensitivity study and performance evaluation of high-performance liquid chromatography-laser-induced fluorescence detection (HPLC-LIF) system assembled in our laboratory for proteomics applications. The limits of Detection (LOD) of several serum proteins have been estimated with this instrument and are found to be much lower compared to other commonly used proteomics techniques like SELDI, MALDI, 2-D-SDS-PAGE, and so forth. Techniques for improving the LOD still further with similar setup are briefly discussed. Using the system, protein profiles of serum in normal, malignant, and premalignant conditions were recorded for different malignancy situations.