Table of Contents
ISRN Analytical Chemistry
Volume 2012, Article ID 674392, 5 pages
http://dx.doi.org/10.5402/2012/674392
Research Article

Development and Validation of Liquid Chromatographic Method for Estimation of Ibuprofen and Famotidine in Combined Dosage Form

Indukaka Ipcowala College of Pharmacy, Phase IV, Beyond GIDC, New Vallabh Vidyanagar, P.B. No. 53, P.O. Vithal Udyognagar, Gujrat-388 121, India

Received 12 January 2012; Accepted 14 February 2012

Academic Editors: V. L. Cebolla, C. Desiderio, D. J. Fletouris, and W. Misiuk

Copyright © 2012 Dimal A. Shah et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

An isocratic, reversed phase-liquid-chromatographic assay method was developed for the quantitative determination of ibuprofen and famotidine in combined-dosage form. A Brownlee C18, 5 μm column with mobile phase containing water : methanol : acetonitrile (30 : 60 : 10, v/v/v) was used. The flow rate was 1.0 mL/min, and effluents were monitored at 264 nm. The retention times of ibuprofen and famotidine were 4.9 min and 6.8 min, respectively. The linearity for ibuprofen and famotidine was in the range of 2–20 μg/mL and 0.1–10 μg/mL, respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity, and robustness. The method was successfully applied to the estimation of ibuprofen and famotidine in combined dosage form.