Figure 5: Activity of Cav1.2 expressed in the absence of β or α 2δ subunits. Shown are (a) epifluorescent images illustrating the predominant PM localization of EYFPN-α 1C (scaling bars, 4 μm) in COS1 cells and (b) superimposed traces of the maximum (black) and (red) scaled to the same amplitude for α 1C/β 2d/α 2δ/CaMex (A), β-free α 1C/α 2δ/CaMex [18] (B) and α 2δ-free α 1C/β 2d/CaMex channel [57] (C).