Table of Contents
ISRN Molecular Biology
Volume 2012, Article ID 706545, 8 pages
http://dx.doi.org/10.5402/2012/706545
Research Article

Increased Phospho-Keratin 8 Isoforms in Colorectal Tumors Associated with EGFR Pathway Activation and Reduced Apoptosis

1Department of Haematology-Oncology, The Queen Elizabeth Hospital, Woodville, SA 5011, Australia
2Physiology Department, School of Medical Sciences, University of Adelaide, Adelaide, SA 5005, Australia
3Flinders Proteomics Laboratory, Department of Human Physiology, Flinders University, Bedford Park, SA 5042, Australia
4Adelaide Proteomics Centre, School of Molecular and Biomedical Science, University of Adelaide, Adelaide, SA 5005, Australia

Received 28 September 2011; Accepted 30 October 2011

Academic Editors: F. Dantzer and S. Iwashita

Copyright © 2012 Georgia Arentz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

We have provided 6 supplementary figures illustrating (1) laser microdissection of tumour section, (2) flow cytometry of Caco2 cells to determine saturating amount of anti-EGFR SC-120 antibody, (3) MALDI-TOF MS spectra showing detection of PS432 after Phos-Trap™ enrichment, (4) Tandem MS analysis showing a mass loss of 98 (a phosphate group) conferring to K8 PS74, (5) Tandem MS analysis identifying K8 PS432 as the most likely residue, (6) 2D western blots confirming the phosphorylated serine residues of K8 isoforms as PS24, PS432 and PS74. Finally, the 2D DIGE method is described in further detail.

  1. Supplementary Material