Table of Contents
ISRN Optics
Volume 2012, Article ID 735231, 13 pages
Research Article

Validation of an XCT/fDOT System on Mice

1CEA, LETI, MINATEC, 17 Rue des Martyrs, 38054 Grenoble Cedex 9, France
2SCANCO Medical AG, Fabrikweg 2, 8306 Brüttisellen, Switzerland
3INSERM U823, Institut Albert Bonniot, 38706 La Tronche Cedex, France
4Institute for Biomedical Engineering, University and ETH Zurich, Wolfgang-Pauli-Strasse 10, 8093 Zürich, Switzerland

Received 12 December 2011; Accepted 22 January 2012

Academic Editors: S. Liu and R. J. Zawadzki

Copyright © 2012 Anne Koenig et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In this paper we present systems for dual modality imaging, combining fluorescence-enhanced diffuse optical tomography and X-ray computed tomography. Fluorescence diffuse optical tomography is carried out in a cylindrical geometry, which ensures optimal sampling and a straight forward integration with the X-ray modality. Specific acquisition protocols and reconstruction software have been developed to this end. The X-ray computed tomography serves two purposes. First, it provides the anatomical information in the registered dual modality images. Second, it provides the actual shape and boundaries of the animal as a priori input to the fluorescence reconstruction algorithm. To evaluate the performance of the optical imaging system, experiments have been conducted on phantoms, mice with inserted fluorescing capillaries, and finally on mice bearing tumors, ex-vivo and in-vivo. Experiments on mice with capillaries inserted in different region of interest, allow estimating the detection limits of fluorophore concentrations. The fluorescence reconstructions are shown to be geometrically consistent with the X-ray images. Finally we demonstrate the capability of the bimodal system to localize real tumours in mice in-vivo. These results show that dual modality fluorescence-enhanced diffuse optical tomography and X-ray computed tomography imaging in cylindrical geometry has a high potential for small animal tumour evolution studies.