Using the external shape derived from the XCT volume to define the excitation source points. (a) Illustration of the ray-driven approach. (b) Selection of the relevant excitation source intersection point for complex shapes. (c) Mesh obtained for a mouse, used for fDOT reconstruction. The nodes of the mesh are displayed as black dots. Sources (red dots) are located at 24 azimuth angles and 12 longitudinal positions. The intensity of fluorescence and excitation light is sampled at the detector positions shown in blue.
