Downregulation in GATA4 and Downstream Structural and Contractile Genes in the db/db Mouse Heart

Table 1

PCR primer sequences.

Gene	Sense primer (5′–3′)	Antisense primer (5′–3′)	Accession no.
ANP	CCTGTGTACAGTGCGGTGTC	CCTAGAAGCACTGCCGTCTC	NM_008725
BNP	CTGAAGGTGCTGTCCCAGAT	GTTCTTTTGTGAGGCCTTGG	NM_008726
IRAP	CAAAGACCGAGCCAACCTGATC	GCTAAAGAGGAACAACCAGCC	NM_172827
GATA	CACTATGGGCACAGCAGCTCC	TTGGAGCTGGCCTGCGATGTC	NM_008092
α-MHC	CTGCTGGAGAGGTTATTCCTCG	GGAAGAGTGAGCGGCGCATCAAGG	NM_001164171
β-MHC	TGCAAAGGCTCCAGGTCTGAGGGC	GCCAACACCAACCTGTCCAAGTTC	NM_080728
CHIP	AGGGCAAGGAGGAAAAGGA	TGGCAATGGCCTCATCATAA	NM_019719
Atrogin1	ACTGGACTTCTCGACTGCCAT	CTCCATCCGATACACCCACAT	AF441120
MURF1	AACACAACCTCTGCCGGAA	AGCCCCAAACACCTTGCA	DQ229108
GLUT4	ACCCTGGGCTCTGTATCCC	CCCTGACCACTGAGTGCAAA	AB008453
NAB1	TGCTGACAAGAAGAGATGAG	TCCTGGTTTCCACAGACTAC	NM_008667
GAPDH	TTCACCACCATGGAGAAGGC	GGCATGGACTGTGGTCATGA	NM_008084

ANP: atrial natriuretic peptide; BNP: brain natriuretic peptide; IRAP: insulin-regulated aminopeptidase; G4: GATA binding protein 4; α-MHC: alpha-myosin heavy chain; α-MHC: beta-myosin heavy chain; CHIP: carboxy terminus of Hsc70-interacting protein; atrogin1: F-box protein 32; MuRF1: muscle RING finger protein 1; GLUT4: glucose transporter protein 4; NAB1: NGF1A-binding protein; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.