Table of Contents
ISRN Oncology
Volume 2012, Article ID 746025, 10 pages
Research Article

Adverse Cell Culture Conditions Mimicking the Tumor Microenvironment Upregulate ABCG2 to Mediate Multidrug Resistance and a More Malignant Phenotype

School of Pharmacy, The Chinese University of Hong Kong, Hong Kong

Received 7 February 2012; Accepted 1 March 2012

Academic Editors: S. Chakrabarty, P. Clavère, N. Fujimoto, and T. T. Trangas

Copyright © 2012 Grace M. Y. Cheng and Kenneth K. W. To. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure legend for Supplementary Figure 1: ABCG2 promoter luciferase reporter gene assay showing that the promoter region harboring the HIF-1α response element is required for the activation of ABCG2 in HCT‐116 human colon cancer cell line. Reporter activity in HCT-116 cells transiently transfected with the various ABCG2 promoter constructs was measured with or without 24-h pretreatment with 2-DG (20 mM) or hypoxia. The mean reporter activity ± SD (firefly/renilla luciferase units [RLU]) from three independent experiments is shown.

  1. Supplementary Figure