Table of Contents
ISRN Dentistry
Volume 2012, Article ID 751920, 5 pages
http://dx.doi.org/10.5402/2012/751920
Research Article

Evaluation of PECAM-1 Gene Polymorphism in Patients with Periodontal Disease and Healthy Individuals

1Periodontics Department, Dental School, Shahid Beheshti University of Medical Sciences, Hamadan, Iran
2Immunology Department, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran
3Dental School, Hamadan University of Medical Sciences, Hamadan, Iran
4Dental School, Shahid Beheshti University of Medical Sciences, Hamadan, Iran

Received 1 December 2011; Accepted 25 December 2011

Academic Editor: J. H. Jeng

Copyright © 2012 Mahdi Kdkhodazadeh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. Our aim in this paper was to investigate the possible genetic association between three Ser563Asn, Leu125Val and Arg670Gly polymorphisms of the PECAM-1 gene and periodontitis. Methods. Genomic DNA was isolated from whole blood of 105 periodontal patient (52 with chronic periodontitis and 53 with aggressive periodontitis) and 101 healthy individuals. Samples were genotyped and analyzed for the three single-nucleotide polymorphisms (SNPs) of PECAM-1 using polymerase chain reaction with sequence-specific primers (PCR-SSPs). Results. A statistically significant difference was found between the genotypic distribution of the Ser563Asn polymorphism in patients with periodontitis compared to controls ( 𝑃 = 0 . 0 2 ). But there were no statistically significant difference between the allele frequencies in the different groups ( 𝑃 = 0 . 0 5 ). The other two polymorphisms did not show a statistically significant difference in their allele and genotype frequencies between the groups. There was no statistically significant difference found for any of the polymorphisms allele and genotype distribution in aggressive and chronic periodontitis either. Conclusions. No significant association was found between the polymorphism tested and the subgroups of periodontitis, further research is still necessary to determine whether this polymorphism can be used as a genetic marker of periodontitis.