Application of a Reliable LC-MS/MS Method for Determination of Rizatriptan in Healthy Subject Samples: Demonstration of Assay Reproducibility by Incurred Sample Reanalysis
Table 3
Stability results for rizatriptan under different conditions.
Stability
Storage condition
Level
Mean stability samplea (ng/mL)
% CV
% change
Bench top stability
Room temperature (24 h)
LQC
0.57
1.6
−5.0
HQC
45.19
1.9
0.4
Processed sample stability (extracted samples)
Auto sampler (4°C, 72 h)
LQC
0.59
1.9
−1.7
HQC
41.70
1.8
−7.3
Processed sample stability (extracted samples)
Room temperature (72 h)
LQC
0.58
2.9
−3.3
HQC
42.13
2.3
−6.4
Freeze and thaw stability
After 6th cycle at −20°C
LQC
0.56
3.8
−6.7
HQC
45.39
2.7
0.9
Long-term stability
79 days at −20°C
LQC
0.60
2.2
0.0
HQC
45.50
1.5
1.1
% Change = ((Mean stability samples − Mean comparison samples)/Mean comparison samples) × 100 LQC: low-quality control; HQC: high-quality control; CV: coefficient of variation.
aMean of six replicates at each level.
