International Scholarly Research Notices / 2012 / Article / Tab 5

Research Article

Application of a Reliable LC-MS/MS Method for Determination of Rizatriptan in Healthy Subject Samples: Demonstration of Assay Reproducibility by Incurred Sample Reanalysis

Table 5

Salient features of the present method in comparison with reported procedures for determination of rizatriptan.

Sr. numberDetection technique; LLOQ
Extraction technique; plasma volume for processing
Retention time; run time; maximum on-column loading at ULOQ per injection volumeOrganic solvent consumption (extraction and chromatography) per sample analysisPostcolumn infusion study; relative matrix effect results (% CV)Incurred sample reanalysis resultsReference

1aLC-MS/MS; 0.1 ng/mLSPE; 1000 μL3.3 min; 4.0 min; 25 ng~4.5 mLNANA[9]
2HPLC; 0.5 ng/mLLLE; 1000 μL5.6 min; 10 min; 10.0 ng~5.0 mLNANA[11]
3LC-MS/MS; 0.05 ng/mL LLE; 100 μL1.039 min; 2.0 min; 2.0 ng~2.5 mLNANA[12]
4LC-MS/MS; 0.05 ng/mLLLE; 500 μL3.73 min; 5.0 min; 6.0 ng~3.5 mLNANA[13]
5LC-MS/MS; 0.1 ng/mLLLE; 100 μL0.94 min; 3.0 min; 125 pg ~4.0 mLNANA[14]
6bLC-MS/MS; 0.5 ng/mLSPE; 1000 μL5.1 min; 7.0 min; 6.25 ng~13.0 mLNANA[15]
7cLC-MS/MS; 1.0 ng/mLSPE; 1000 μL3.48 min; 5.0 min; 25 ng~3.0 mLNANA[16]
8LC-MS/MS; 0.2 ng/mLLLE; 300 μL1.71 min; 3.0 min; 187.5 pg~5.0 mLYes; 0.5 to 4.6% for 8 lots of plasma% change from 11.8 to −10.2%PM

aIn plasma of dogs; balong with sumatriptan; cin human serum along with zolmitriptan, naratriptan, and sumatriptan; LLOQ: lower limit of quantitation; ULOQ: upper limit of quantitation; SPE: solid phase extraction; LLE: liquid-liquid extraction; NA: data not available; PM: present method.

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