Figure 2: Detection of IFNγ induced cell death by flow cytometry and western blot. (a and b) Apoptotic cell detection via flow cytometry of FLOH1, RH30, TE671, and HT29 cell lines after IFNγ treatment; cells were incubated for different periods of time with 100 ng/mL IFNγ in starvation media with 1% FCS and stained with propidium iodide and annexin V; (a) shows proportion of propidium iodide positive cells after IFNγ treatment at different time points; (b) reflects the complete flow cytometry data for HT29 control cell lines; (c) western blot analysis of caspase 8 cleavage 24 h after IFNγ treatment; β-actin serves as loading control.