Figure 5: IFNγ treatment does not alter protein expression of MHCII by RMS cells. (a, b) Induction of MHCII and MHCI in RMS cell lines and (d) in HT29 control cells 48 h after IFNγ treatment (100 ng/mL); grey filled histograms represent expression levels after incubation with IFNγ; open histograms represent expression levels without IFNγ incubation; broken lines represent the negative control (secondary antibody). In (d), the broken lines overlap with the lines of the open histograms; (c) western Blot Analysis of CIITA induction 48 h after IFNγ treatment (100 ng/mL); β-actin serves as loading control.