Design and Development of Degradable Polyethylenimines for Delivery of DNA and Small Interfering RNA: An Updated Review

Figure 12

In vitro transfection efficiency of PMT/DNA complexes with or without serum. The same amount of pGL3 plasmids were complexed with PMT, PEI, and Lipofectamine 2000 and delivered to (a) A549 cells, (b) N2a cells, and (c) HeLa cells without serum. (d) The PMT and PEI/pGL3 complexes were transfected in A549 cells in the presence of various amount of serum. Luminescence was measured 24 h after transfection and normalized with the amount of protein. (𝑛=3, error bar represents standard deviation; *𝑃<0.1, **𝑃<0.05, ***𝑃<0.01, one-way ANOVA compared to that of PEI/DNA complexes (a)–(c) and control (d)) [56].

(a)

(b)

(c)

(d)