Table of Contents
ISRN Analytical Chemistry
Volume 2012, Article ID 815353, 6 pages
http://dx.doi.org/10.5402/2012/815353
Research Article

Normal and Reversed-Phase HPTLC Methods for Simultaneous Estimation of Telmisartan and Metoprolol Succinate in Pharmaceutical Formulation

Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, 425 405 Shirpur, Maharashtra, India

Received 23 August 2011; Accepted 13 September 2011

Academic Editors: T. Bolanca, R. K. Jyothi, T. Macko, and W. Misiuk

Copyright © 2012 Prajakta S. Nawale et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Two simple precise normal-phase (Method I) and reversed-phase (Method II) HPTLC/densitometry method have been developed for simultaneous determination of telmisartan and metoprolol succinate in bulk and in combined tablet formulation. Method I was developed with aluminium plates precoated with silica gel 60F254S, and toluene : propanol : methanol : triethylamine (8 : 1 : 1 : 0.5 v/v) was used as as mobile phase. Method II was carried out using aluminium coated with RP-18 silica gel 60 F254S HPTLC plates using methanol : water : triethylamine (6 : 4 : 0.5 v/v) as mobile phase. Both analyses were scanned with a densitometer at 242 nm. In Method I, good separation and resolution of drugs were achieved with 𝑅 𝑓 values 0 . 4 5 Β± 0 . 0 2 (telmisartan) and 0 . 7 0 Β± 0 . 0 2 (metoprolol), while in Method II, telmisartan and metoprolol showed 𝑅 𝑓 values 0 . 5 5 Β± 0 . 0 2 and 0 . 4 1 Β± 0 . 0 2 , respectively. These methods can be used in routine pharmaceutical analysis.