Table of Contents
ISRN Analytical Chemistry
Volume 2012, Article ID 816349, 7 pages
Research Article

Application of Ion-Association Titration for the Assay of Cyproheptadine Hydrochloride in Pharmaceuticals

Department of Chemistry, University of Mysore, Manasagangotri, Mysore 570006, India

Received 23 December 2011; Accepted 19 January 2012

Academic Editors: H. Alemu, M. Mazloum-Ardakani, A. Orte, and B. Rittich

Copyright © 2012 Madihalli S. Raghu and kanakapura Basavaiah. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Two simple and rapid titrimetric methods are described for the determination of cyproheptadine hydrochloride (CPH) in pharmaceuticals. The proposed methods are based on the solvent extraction-titration of CPH with two ion association reagents, sodium lauryl sulphate (SLS), (method A), and tetraphenylborate (TPB), (method B). In method A, SLS was used as titrant and the titration was carried out in the presence of dilute sulphuric acid and chloroform using dimethyl yellow as indicator, whereas, in method B, the titrant was TPB and the titration was done in Walpole buffer of pH 4.5 and 1, 2-dichloroethane using tetrabromophenolphthalein ethyl ester as indicator. The proposed procedures give sharp end points as the color of the organic phase changes from yellow to pink in method A, and from red-violet to yellow in method B. The methods are applicable over the ranges of 1.0–20.0 and 2.0–9.0 mg of CPH for method A and method B, respectively. The accuracy and precision of the methods are good. The methods were applied successfully to the determination of CPH in tablets, and the results were in agreement with the label claim and those of the reference method.