Morphological Changes of Mammalian Nucleoli during Spermatogenesis and Their Possible Role in the Chromatoid Body Assembling

Figure 2

(a) Percentage of germ cells (spermatogonia and round spermatids) of Oryctolagus cuniculus that possessed 1 to 4 nucleoli. The letters represent a statistically difference in the number of nucleoli in each cell type, and the asterisk represents statistically difference between both cell types (LSD, 𝑃<0.05). The means (±SD) of the number of spermatogonia and round spermatids possessing 1, 2, 3, or 4 nucleoli were, spermatogonia: 1 nucleolus 61.33 (±13.72); 2 nucleoli 34.5 (±3.67); 3 nucleoli 17 (±3.42); 4 nucleoli 9.33 (±6.06). Round spermatids: 1 nucleolus 83.16 (±14.51); 2 nucleoli 36.5 (±11.32); 3 nucleoli 16.66 (±3.97); 4 nucleoli 6.33 (±2.94). B: areas (nm²) of the nuclei and nucleoli of spermatogonia and round spermatids. The asterisk (*) represents a statistically significant difference between the cell types (independent t test, 𝑃<0.05).

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