Table of Contents
ISRN Chromatography
Volume 2012, Article ID 836132, 5 pages
Research Article

An Isocratic Method for Quantification of Valproic Acid and Its Related Impurities Using Ion Pair Reagent by Ultraperformance Liquid Chromatography

National Facility for Drug Discovery, Department of Chemistry, Saurashtra University, Rajkot 360005, Gujarat, India

Received 26 October 2012; Accepted 15 November 2012

Academic Editors: S. De Marino and X. Guan

Copyright © 2012 Rakshit Thakkar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A selective ultraperformance liquid chromatographic (UPLC) method for the quantification of valproic acid and its known related impurities using ion pair reagent has been developed. The method includes reversed-phase Acquity HSS T3 column with 100 mm × 2.1 mm i.d. and 1.7 μ particle size. The mobile phase consists of acetonitrile, 5 mM 1-hexanesulphonic acid sodium salt, flow rate is 0.6 mL/min, and UV detection is performed at 215 nm. A system suitability test (SST) was developed to govern the quality of the separation. The developed method has been validated further with respect to linearity, accuracy, precision, selectivity, LOD, LOQ, and Robustness.