Table 4: Summary of validation results observed by the proposed method.

ParameterPA2-EPA2-IsoPPAVPA

LOD ( g/mL)0.50.50.50.5
LOQ ( g/mL)2.02.02.02.0
Accuracy (%)99.12–99.8098.27–101.1100.01–101.998.56–101.35
Precision (RSD, %)
 Method precision0.611.210.901.07
 Intermediate precision0.910.330.561.34
Linearity
 Range ( g/mL)50–40050–40050–40050–400
 Correlation coefficient0.9980.9970.9990.995
 Slope211.6238.6250.4321.8
 Intercept−121712028−8400−7153
 Theoretical plate 35426 21329 17200 31128
 Asymmetry1.061.231.021.31