Research Article
An Isocratic Method for Quantification of Valproic Acid and Its Related Impurities Using Ion Pair Reagent by Ultraperformance Liquid Chromatography
Table 4
Summary of validation results observed by the proposed method.
| Parameter | PA | 2-EPA | 2-IsoPPA | VPA |
| LOD (g/mL) | 0.5 | 0.5 | 0.5 | 0.5 | LOQ (g/mL) | 2.0 | 2.0 | 2.0 | 2.0 | Accuracy (%) | 99.12–99.80 | 98.27–101.1 | 100.01–101.9 | 98.56–101.35 | Precision (RSD, %) | | | | | Method precision | 0.61 | 1.21 | 0.90 | 1.07 | Intermediate precision | 0.91 | 0.33 | 0.56 | 1.34 | Linearity | | | | | Range (g/mL) | 50–400 | 50–400 | 50–400 | 50–400 | Correlation coefficient | 0.998 | 0.997 | 0.999 | 0.995 | Slope | 211.6 | 238.6 | 250.4 | 321.8 | Intercept | −1217 | 12028 | −8400 | −7153 | Theoretical plate |
35426 |
21329 |
17200 |
31128 | Asymmetry | 1.06 | 1.23 | 1.02 | 1.31 |
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