Isolation of Alcohol Dehydrogenase cDNA and Basal Regulatory Region from Metroxylon sagu

Table 1

Nucleotide sequence of primers used in genomic walking. Overhanging primers (OHP) with KpnI, PstI, and SacI overhang sequence indicated in italic bold adaptor primer (AP), nested primer (NP), and Adh gene specific primer (GSP4).

Primer name	Sequence
OHP KpnI	5′-GAATTCGAGCTCGCCCGGGATCCTCTAGAGTAC -3′
OHP PstI	5′-GAATTCGAGCTCGCCCGGGATCCTCTAGATGCA -3′
OHP SacI	5′-GAATTCGAGCTCGCCCGGGATCCTCTAGAAGCT -3′
AP	5′-GAATTCGAGCTCGCCCGGGAT-3′
NP	5′-GCTCGCCCGGGATCCTCTAGA-3′
GSP4	5′-GCCCTTAGCTTCCCAGAAGT-3′