Table of Contents
ISRN Analytical Chemistry
Volume 2012 (2012), Article ID 859473, 17 pages
Review Article

Application of Different Analytical Techniques and Microbiological Assays for the Analysis of Macrolide Antibiotics from Pharmaceutical Dosage Forms and Biological Matrices

Food and Drug quality Control Laboratory, Food, Medicine and Health Care Administration and Control Authority of Ethiopia, Addis Ababa 5681, Ethiopia

Received 7 March 2012; Accepted 2 May 2012

Academic Editors: I. Lavilla and I. Zhukov

Copyright © 2012 Lantider Kassaye Bekele and Getachew Genete Gebeyehu. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Macrolides are a group of drugs whose activity stems from the presence of a macrolide ring, a large macrocyclic lactone ring to which one or more deoxy sugars may be attached. They are produced by Streptomyces species and used primarily against gram-positive bacteria. The determination of antibiotics, including macrolides, is mainly carried out by microbiological assays. However, microbiological assays tended to lack specificity. And hence to overcome this problem, lots of chemical and instrumental methods have been developed to determine macrolides separately as well as simultaneously. Different chromatographic, spectrophotometric, and electrochemical methods used for the determination of macrolides have been reviewed in this paper.