Application of Different Analytical Techniques and Microbiological Assays for the Analysis of Macrolide Antibiotics from Pharmaceutical Dosage Forms and Biological Matrices
Table 3
Buffer solutions, solvents, microorganisms, and incubation temperature employed in diffusion types of microbial assay (BP 2009 and USP 2000).
Macrolide antibiotics
Reference used
Solvent used
Microorganisms used
Medium and final pH
Incubation temperature (°C)
Erythromycin estolate
Erythromycin CRS
Methanol
Bacillus pumilus
Peptone (6 g) + pancreatic digest of casein (4 g) + beef extract (1.5 g) + yeast extract (3 g) + glucose monohydrate (1 g) + agar (15 g) + water to produce 1000 mL, pH 7.9
30–37
Erythromycin stearate
Erythromycin CRS
Methanol
Bacillus subtilis
Peptone (6 g) + pancreatic digest of casein (4 g) + beef extract (1.5 g) + yeast extract (3 g) + glucose monohydrate (1 g) + agar (15 g) + water to produce 1000 mL, pH 8.0
30–37
Spiramycin
Spiramycin CRS
Methanol
Bacillus subtilis
Peptone (6 g) + pancreatic digest of casein (4 g) + beef extract (1.5 g) + yeast extract (3 g) + glucose monohydrate (1 g) + Agar (15 g) + water to produce 1000 mL, pH 8.0
30–32
Tylosin
Tylosin CRS
2.5% v/v solution in 0.1 N phosphate buffer solution (pH 7)
Micrococcus flavus
Peptone (6 g) + pancreatic digest of casein (4 g) + beef extract (1.5 g) + yeast extract (3 g) + glucose monohydrate (1 g) + Agar (15 g) + water to produce 1000 mL, pH 8.0
