Application of Different Analytical Techniques and Microbiological Assays for the Analysis of Macrolide Antibiotics from Pharmaceutical Dosage Forms and Biological Matrices

Table 4

Buffer solutions, solvents, microorganisms, and incubation temperature employed in turbidimetric types of microbial assay ((BP 2009 and USP 2000).

Macrolide antibiotics	Reference used	Solvent used	Microorganisms used	Medium and final pH	Incubation temperature (°C)
Erythromycin estolate and erythromycin ethyl succinate	Erythromycin; CRS	Methanol	Klebsiella pneumoniae	Heart extract (1.5 g) + yeast extracts (1.5 g) + peptone-casein (5 g) + glucose monohydrate (1 g) + sodium chloride (3.5 g) + dipotassium hydrogen phosphate (3.68 g) + potassium dihydrogen phosphate (1.32 g) + potassium nitrate (2 g) + water to produce 1000 mL, pH 7	35–37
Erythromycin  stearate	Erythromycin; CRS	Methanol	Staphylococcus aureus	Peptone (6 g) + Beef extract (1.5 g) + yeast extract (3 g) + sodium chloride (3.5 g) + glucose monohydrate (1 g) + dipotassium hydrogen phosphate (3.68 g) + potassium dihydrogen phosphate (1.32 g) + water to produce 1000 mL, pH 7	35–37
Spiramycin	Spiramycin; CRS	Methanol	Staphylococcus aureus	Peptone (6 g) + beef extract (1.5 g) + yeast extract (3 g) + sodium chloride (3.5 g) + glucose monohydrate (1 g) + dipotassium hydrogen phosphate (3.68 g) + potassium dihydrogen phosphate (1.32 g) + water to produce 1000 mL, pH 8	35–37
Tylosin	Tylosin CRS	2.5%v/v solution in; 0.1N phosphate buffer solution; (pH 7)	Staphylococcus aureus	Peptone (6 g) + beef extract (1.5 g) + yeast extract (3 g) + sodium chloride (3.5 g) + glucose monohydrate (1 g) + dipotassium hydrogen phosphate (3.68 g) + potassium dihydrogen phosphate (1.32 g) + water to produce 1000 mL, pH 8	37