Table of Contents
ISRN Ophthalmology
Volume 2012, Article ID 860819, 6 pages
Clinical Study

Reproducibility of Retinal Nerve Fiber Layer Measurements with Manual and Automated Centration in Healthy Subjects Using Spectralis Spectral-Domain Optical Coherence Tomography

1Department of Ophthalmology and MS Clinic, Department of Neurology, University of British Columbia, Vancouver, BC, Canada V5Z 3N9
2MS Clinic, Department of Neurology, The University of British Columbia, Vancouer, BC, Canada V6T 2B5
3Departments of Clinical Neurosciences and Surgery, Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada T2N 1N4
4Jules-Gonin Eye Hospital, University of Lausanne, 1015 Lausanne, Switzerland

Received 7 May 2012; Accepted 5 June 2012

Academic Editors: M. P. Shanmugam and K. Wu

Copyright © 2012 Alex P. Lange et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. The aim of this study was to test the reproducibility of the Heidelberg Spectralis SD-OCT and to determine if provided software retest function for follow-up exam is superior to manual centration. Design. Prospective, cross-sectional study. Participants. 20 healthy subjects. Methods. All subjects underwent SD-OCT testing to determine retinal nerve fiber layer (RNFL) measurements sequentially on two different days and with two different centration techniques. Within-subject standard deviation, coefficient of variation, and intraclass correlation coefficient were used to assess reproducibility. Results. RNFL measurements showed high reproducibility, low within-subject standard deviation (1.3), low coefficient of variation (0.63%), and low intra-class correlation coefficient (0.98 (95% CI 0.97–0.99)) in the automated centration and manual centration groups for average RNFL Thickness. Quadrants showed slightly higher variability in the manual group compared to the automated group (within-subject standard deviation 2.5–5.3 versus 1.1–2.4, resp.). Conclusions. SD-OCT provides high-resolution RNFL measurements with high reproducibility and low variability. The re-test function allows for easier recentration for longitudinal examinations with similar results in average RNFL, but less variability in quadrant RNFL. SD-OCT high reproducibility and low variability is a promising fact and should be further evaluated in longitudinal studies of RNFL.