Novel Reagents for the Spectrophotometric Determination of Isoniazid

Table 1

Analytical parameters.

	EPI	HPC
𝜆max  nm	405	402
Beer’s law limit (μg mL^−1)	2.00–22.00	20.00–120.00
Molar absorptivity (L mol^−1 cm^−1)	0.51 × 10^4	0.10 × 10^4
Sandell’s sensitivity (μg cm^−2)	0.027	0.134
Regression equation*	𝑌=𝑎+𝑏𝑋	𝑌=𝑎+𝑏𝑋
Slope (𝑏)	0.0336	0.0064
Intercept (𝑎)	0.022	0.018
Correlation coefficient (𝑟)	0.9910	0.9979
Limit of Detection** (μg mL^−1)	1.500	5.150
Limit of Quantitation** (μg mL^−1)	4.545	15.620

* 𝑌 is the absorbance and 𝑋 is the concentration in μg mL⁻¹.
** Calculated using ICH guidelines.