A Novel Solid Substrate Room Temperature Phosphorimetry for the Determination of Trace Cytochrome C and Forecast of Human Diseases

Table 1

The measurement condition.

The measurement condition		The Δ𝐼p of Cyt C-F-ol-tween-80 system	Optimal
F-ol (×10^−4 mol L^−1); (mL)	1.0, 0.10, 0.010, 0.0010; 0.50, 1.00, 1.50, 2.00, 2.50	21.2, 34.1, 15.7, 7.2; 11.6, 33.7, 26.1, 19.0, 17.5	1.0×10^−5 mol L^−1; 1.00 mL
tween-80 (1%); (mL)	0.5, 1.0, 2.0, 3.0, 5.0; 1.00, 1.50, 2.00, 2.50, 3.00, 4.00	8.22, 11.9, 34.5, 28.7, 23.5; 8.6, 20.9, 33.5, 28.3, 26.1, 17.8	2.0%; 2.00 mL
Pb^2+  (10^−4 mol L^−1)	0.10, 0.25, 0.75, 1.00, 1.20	14.8, 16.2, 20.5, 33.8, 20.9	1.00 mol L^−1
pH of the system	1.99, 3.42, 4.98, 5.76, 6.08, 6.63, 7.42, 8.31, 10.05	4.6, 17.4, 33.2, 33.9, 34.1, 33.6, 33.4, 21.4, 12.9	4.98–7.42
Reaction temperature (°C)	30, 40, 50, 60, 70, 80, 90	10.5, 23.7, 34.3, 24.0, 15.6, 12.3, 5.2	50°C
Reaction time (min)	5, 10, 15, 20, 25	5.1, 13.9, 33.5, 25.6, 19.1	15 min
Temperature for drying (°C)	50, 60, 70, 80, 90, 95	10.9, 13.1, 18.3, 26.1, 33.6, 21.7	90°C
Time for drying (min)	0.5, 1.0, 1.5, 2.0, 2.5, 3.0	16.4, 20.2, 22.9, 27.7, 33.0, 28.2	2 min
Standing time (min)	10, 20, 30, 40, 50, 60, 70, 80	33.1, 33.7, 33.9, 34.0, 34.2, 33.8, 21.4, 12.9	10–60 min