Figure 2: Effect of tryptase on the VEGF, Flt-1, and Flk-1 protein levels in HDMECs with or without APC366. HDMECs were treated with different concentrations of tryptase for 18 h in the absence or presence of APC366 (250 μg/mL). The protein levels of VEGF (a), Flt-1 (b), and Flk-1 (c) were determined by Western blot and normalized to GAPDH. The heparin control was also analyzed. 𝑃 < 0 . 0 5 compared to the group of nonaddition. # 𝑃 < 0 . 0 5 compared to the group only treated with tryptase at the same concentration.