Affinity and Matrix Effects in Measuring Fish Plasma Vitellogenin Using Immunosorbent Assays: Considerations for Aquatic Toxicologists

<table>Concept map of interactions between antibody specificity and matrix composition in determining likely accuracy of calculated vitellogenin concentrations for a given plasma sample. Predictions of accuracy of an ELISA using a specific antibody and similar or different matrices follow the concepts of antibody affinity and likelihood that plasma proteins will interfere with the recognition of the target molecule by the antibody.</table>

International Scholarly Research Notices

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Figure 1

Figure 1: Affinity and Matrix Effects in Measuring Fish Plasma Vitellogenin Using Immunosorbent Assays: Considerations for Aquatic Toxicologists 