Affinity and Matrix Effects in Measuring Fish Plasma Vitellogenin Using Immunosorbent Assays: Considerations for Aquatic Toxicologists

Table 2

Recovery of purified vitellogenin added to dilution buffer and measured via competitive polyclonal ELISA. Multiple dilutions of purified vitellogenin were prepared in PBS/1% BSA and incubated with antivitellogenin rabbit polyclonal antiserum prior to exposure to microtiter plates coated with the identical purified vitellogenin. Recovery is based on purified vitellogenin concentration of 600 μg/mL.

Recovery effort (date)	Repeated vitellogenin analysis	Vitellogenin concentration	Percent of recovery
		mean ± stand. err.
3/21/2010	642 μg/mL	703.4 ± 32.3 μg/mL	117%
	618 μg/mL
	788 μg/mL
	753 μg/mL
	716 μg/mL
7/9/2010	492 μg/mL	515 ± 7.2 μg/mL	86%
	520 μg/mL
	538 μg/mL
	530 μg/mL
	510 μg/mL
	500 μg/mL
3/11/2011	498 μg/mL	463.2 ± 9 μg/mL	77%
	421 μg/mL
	436 μg/mL
	504 μg/mL
	450 μg/mL
	476 μg/mL
	467 μg/mL
	453 μg/mL
	462 μg/mL