Table of Contents
ISRN Pharmacology
Volume 2012, Article ID 948792, 8 pages
http://dx.doi.org/10.5402/2012/948792
Research Article

Influence of Alternanthera brasiliana (L.) Kuntze on Altered Antioxidant Enzyme Profile during Cutaneous Wound Healing in Immunocompromised Rats

1Department of Veterinary Pharmacology & Toxicology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati 781022, India
2Department of Veterinary Pathology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati 781022, India
3Department of Veterinary Medicine, Public Health & Hygiene, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati 781022, India
4Department of Veterinary Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati 781022, India
5Department of Livestock Production and Management, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati 781022, India
6Department of Biochemical Pharmacology, Defence Institute of Physiology and Allied Sciences, New Delhi 110054, India

Received 30 April 2012; Accepted 10 June 2012

Academic Editors: G. M. Campo and D. K. Miller

Copyright © 2012 Chandana Choudhury Barua et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Alternanthera brasiliana (L.) Kuntze (Amaranthaceae) is a herbaceous plant used against inflammation, cough, and diarrhea in Brazilian popular medicine. In our preliminary study, promising wound healing activity of methanol extract of leaves of A. brasiliana (MEAB) was observed in normal excision and incision wound models. Therefore, the present study was designed to investigate the wound healing activity along with the antioxidant enzyme profile during cutaneous excision immunocompromised wound after topical application of 5% w/w ointment of MEAB in rats. Immunocompromised state was induced by pretreatment with hydrocortisone (HC) at 40 mg/kg body weight (i.m.) in male rats. Following one-week pretreatment with HC, wounds were created. The vehicle, 5% (w/w) ointment of MEAB, or standard drug (Himax) was applied topically twice daily. Healing potential was evaluated by the rate of wound contraction, estimation of enzymatic and nonenzymatic antioxidants like catalase, SOD, GSH, protein, vitamin C, and hydroxyproline content, which was supported by histopathological study on the 8th day following wounding. There was significant increase in the enzymatic and nonenzymatic antioxidant parameters in the extract-reated group as compared to control group. Histopathological study revealed collagen deposition, fibroblast proliferation, angiogenesis, and development of basement membrane in A. brasiliana group. The results of the present investigation revealed significant wound healing activity of MEAB.