Table of Contents
ISRN Analytical Chemistry
Volume 2012, Article ID 963276, 6 pages
http://dx.doi.org/10.5402/2012/963276
Research Article

Liquid Chromatographic Method for the Quantification of Antipsychotic Agent Iloperidone in Pharmaceutical Formulation

1Indukaka Ipcowala College of Pharmacy, Beyond GIDC Phase V, Vithal Udyognagar, New Vallabh Vidyanagar, Gujarat, Anand 388121, India
2Sun Pharmaceutical Industries Ltd, Formulation and Development Department, Baroda 390020, Gujarat, India

Received 14 December 2011; Accepted 9 January 2012

Academic Editor: T. Macko

Copyright © 2012 Usmangani K. Chhalotiya et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Liquid chromatographic method for the quantitative determination of iloperidone in the pharmaceutical dosage form was developed. A reversed phase column with mobile phase containing 0.02 M potassium dihydrogen phosphate: methanol: acetonitrile (55 : 25 : 20, v/v/v, pH 4 adjusted with phosphoric acid) was used. The flow rate was 1.0 mL min−1, and effluents were monitored at 230 nm. The retention time of iloperidone was 11.6 min. Iloperidone stock solutions were subjected to acid and alkali hydrolysis, chemical oxidation, and dry heat degradation. The degraded product peaks were well resolved from the pure drug peak with significant difference in their retention time values. Stressed samples were assayed using developed LC method. The proposed method was validated with respect to linearity, accuracy, precision, and robustness. The method was successfully applied to the estimation of iloperidone in tablet dosage forms.