Table of Contents
ISRN Chromatography
Volume 2012, Article ID 970685, 7 pages
Research Article

Using Size-Exclusion Chromatography to Monitor Variations in the Sizes of Microwave-Irradiated Gold Nanoparticles

Department of Applied Chemistry, National University of Kaohsiung, No. 700 Kaohsiung University Road, Nan Tzu District, Kaohsiung 811, Taiwan

Received 13 November 2012; Accepted 30 November 2012

Academic Editors: M. C. Bruzzoniti, L. Chen, J. Matysiak, and L. Membrado

Copyright © 2012 Fu-Ken Liu. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Size-exclusion chromatography (SEC) was used to evaluate gold nanoparticles (Au NPs) for variations in their sizes after microwave (MW) irradiation, with the eluted NPs monitored through diode array detection to reveal their surface plasmon absorptions. The sizes of citrate-capped Au NPs decreased upon increasing the MW irradiation temperature, consistent with digestive ripening of these NPs under the operating conditions. In contrast, Au NPs capped with sodium dodecyl sulfate increased in size upon increasing the MW irradiation temperature, consistent with Ostwald ripening. When the Au NPs were capped with 3A-amino-3A-deoxy-(2AS,3AS)- -cyclodextrin (H2N- -CD), however, their dimensions were barely affected by the MW irradiation temperature, confirming that H2N- -CD is a good stabilizer against MW irradiation. Therefore, SEC—with its short analysis times, low operating costs, automated operation, and in situ analysis—has great potential for use in the rapid monitoring of NPs subjected to treatment under various MW irradiation conditions.