Glycosaminoglycans Modify Elastase Action In Vitro and Enhance Elastase-Induced Cell Death in Cultured Fibroblasts

Table 1

Effect of GAGs on the hydrolysis of MeOSuc-Ala-Ala-Pro-Val-pNA by HNE.

GAG	𝑘cat (s^−1)	𝐾m (mM)	𝑘cat/𝐾m (10^3 M^−1 s^−1)
No GAG	4.73 ± 0.38	0.854 ± 0.001	5.54
Hep	5.55 ± 0.58	1.11 ± 0.08	5.00
KS	3.75 ± 0.18	0.614 ± 0.001	6.11
C4S	3.20 ± 0.03	0.681 ± 0.004	4.70
HS	1.95 ± 0.12	0.468 ± 0.021	3.31
DS	1.83 ± 0.69	0.574 ± 0.088	3.19
C6S	1.49 ± 0.33	0.653 ± 0.001	2.28

HNE (6.0 nM) in 200 mM Tris buffer pH 8.0 was preincubated for 5 min at 37°C with or without GAGs (300 nM). After that, MeOSuc-Ala-Ala-Pro-Val-pNA (0.10 to 2.5 mM) was added, and the hydrolysis was followed by the absorbance in 405 nm. Hep: heparin; HS: heparan sulfate; KS: keratan sulfate; C4S: chondroitin 4-sulfate; C6S: chondroitin 6-sulfate; DS: dermatan sulfate.