Figure 2: The expression of miR-155 was induced by Tax in T cells. Tax-inducible cell line, JPX-9 cells were cultured with CdCl2 (20 μM) for indicated time periods. (a) Tax expression in CdCl2-treated JPX-9 cells. Cell lysates were prepared from CdCl2-treated JPX-9 cells at the indicated time points. Tax protein expression after CdCl2 treatments was determined by western blot. Actin protein expression served as a loading control. (b) miR-155 expression was analyzed by real-time RT-PCR. miR-155 expression is shown as a fold induction relative to the values measured at 0 h. Values are the mean ± SD from three separate experiments.