Table of Contents
ISRN Dentistry
Volume 2012, Article ID 989781, 5 pages
http://dx.doi.org/10.5402/2012/989781
Research Article

An Assessment of Antibacterial Activity of Four Endodontic Sealers on Enterococcus faecalis by a Direct Contact Test: An In Vitro Study

1Department of Conservative Dentistry and Endodontics, RIMS Government Dental College, Andhra Pradesh, Kadapa 516004, India
2Department of Conservative Dentistry and Endodontics, Narayana Dental College and Hospital, Andhra Pradesh, Nellore 524003, India
3Department of Oral Medicine & Radiology, Narayana Dental College and Hospital, Andhra Pradesh, Nellore 524002, India
4Department of Conservative Dentistry & Endodontics, KLR's Lenora Institute of Dental Sciences, Andhra Pradesh, Rajahmundry 533101, India
5Department of Pharmacology, Narayana Medical College and Hospital, Andhra Pradesh, Nellore 524002, India
6Department of Pedodontics, Narayana Dental College and Hospital, Andhra Pradesh, Nellore 524002, India
7Department of Orthodontics, Narayana Dental College and Hospital, Andhra Pradesh, Nellore 524002, India

Received 15 April 2012; Accepted 29 May 2012

Academic Editors: D. Schwartz-Arad and M. Tanomaru-Filho

Copyright © 2012 Lavanya Anumula et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim. To evaluate the antibacterial activity of four endodontic sealers on Enterococcus faecalis by a direct contact test. Material and Methods. Enterococcus faecalis was used as a test organism. Direct contact test which is based on measuring the effect of close contact between test bacteria and tested material on the kinetics of bacterial growth was performed to overcome the disadvantages of agar diffusion test. The sealers tested were zinc oxide eugenol-based sealer, glass-ionomer-based sealer, polydimethyl-siloxane-based sealer, and urethane dimethacrylate resin-based sealer. Data was collected by recording the optical density with the help of a spectrophotometer. Results. The sealers exhibited different inhibitory effects. The results obtained were subjected to statistical analysis by Kruskal Wallis analysis of variance and Dunn's multiple comparison test. Group comparison showed very highly significant difference between the groups. Conclusion. Zinc oxide eugenol-based sealer was the most effective and urethane dimethacrylate resin-based sealer was the least effective against Enterococcus faecalis, whereas glass-ionomer-based and polydimethyl-siloxane-based sealers were effective only for a short period. Inhibition of the bacterial growth is related to the direct contact of the microorganism with the sealer.