Review Article
The Food Additive Polyglycerol Polyricinoleate (E-476): Structure, Applications, and Production Methods
Table 4
Coupling parameters for the immobilization of
Candida rugosa lipase in different supports [
63].
| Support | Immobilization method | Immobilized protein (%) | Enzyme loading (mg E/g support) |
| Biolita L2.7 | Adsorption | — | — | Covalent binding | 0.64 | 0.48 |
| Biolita P3.5 | Adsorption | — | — | Celite R-643 | 27.60 | 4.14 | Chromosorb W | — | — |
| Nonporous glass 425–600 μm | Covalent binding | 0.89 | 0.67 | Nonporous glass 91–107 μm | — | — | PG 75-400 | 41.73 | 31.30 | PG 700-400 | 28.65 | 21.49 | PG 1000-400 | 22.83 | 17.12 |
| Cationic exchange resin Dowex 50 × 8 |
Adsorption | — | — | Anionic exchange resin Lewatit MonoPlus MP 64 | 47.20 | 7.08 |
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