Low-Frequency Mechanical Stimulation Modulates Osteogenic Differentiation of C2C12 Cells
Figure 2
Osteogenic medium induces bone-like differentiation of C2C12 cells. RT-qPCR results for fold changes in Runx2, Osx, Alp, Col1a1, Osteopontin, bone Gla protein, and OPG expression after 6 hours (black bars), 24 hours (grey bars), and 48 hours (white bars), normalized to time zero controls (prior to exposure to osteogenic medium). Mean ± SEM, ; *represents a significant () difference between BMP-2-treated and untreated cultures. Inset: cells treated with BMP-2 stained strongly positive for ALP activity (purple) after 48 hours. Scale bar represents 10 mm.
